chicken polyclonal anti flag  (AvesLabs)

Journal: Virology

Article Title: The physical interactome between Peregrinus maidis proteins and the maize mosaic virus glycoprotein provides insights into the cellular biology of a rhabdovirus in the insect vector.

doi: 10.1016/j.virol.2022.10.002

Figure Lengend Snippet: Fig. 3. Co-immunoprecipitation of CypA or ApoLpIII and MMV G using anti- FLAG affinity gel shows that MMV G interacted with Cyclophilin A and ApoL pIII. Myc-tagged Pm proteins, CypA or ApoLpIII were co-immunoprecipitated with MMV G-FLAG using anti-FLAG affinity gel (Biotool, Huoston, TX).

Article Snippet: After incubation, the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 μl of the resin with 5x SDS loading buffer and the proteins were detected by Western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000, Sta.

Techniques: Immunoprecipitation